LA Phosphate Incorporation in Brain

LA Phosphate Incorporation in Brain Phosphoinositides *

To test this hypothesis, we have investigated the incorporation of radioactive inorganic phosphate into these phospholipids by rabbit brain slices. If the biosynthesis proceeds as formulated, I --f II + III, the following results can be predicted. Before equilibration of the labeled phosphorus, the phosphate groups in position 4 of II and positions 4 and 5 of 111 must have a higher radioactivit...

Brain phosphatidic acid and polyphosphoinositide formation in a broken cell preparation: regional distribution and the effect of age.

The effect of age on phosphate incorporation into phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid (PA) was studied. Lysed crude synaptosomal fractions of different brain regions of 3-month-old and 32-month-old Brown Norway rats were used. The brain regions tested were the hippocampus, frontal cortex, occipital/parietal cortex, entorhina...

Acetylcholine and the exchange of inositol and phosphate in brain phosphoinositide.

Previous studies showed that acetylcholine, in the presence of cserine, stimulated the incorporation of P3* into the phospholipidcs in slices of guinea pig brain cortex (2, 3). The major stimulation occurred in phosphoinositidc and in phosphatidic acid, with a lesser stimulation in phosphatidyl choline (3). The incorporation of glycerol-l-U4 into the total glycerophosphatide fraction was not in...

Characterization of biophysical properties of single chloride channel in rat brain mitochondrial inner membrane by channel incorporation into bilayer lipid membrane

Introduction: Recent studies have shown the presence of Cl- channels in heart and liver mitochondrial membranes. In this work, we have characterized the functional profile of a Cl- channel from rat brain mitochondria. Methods: After removing and homogenizing the rat brain, the supernatant was separately centrifuged in MSEdigitonin, H2O and Na2CO3 and mitochondrial inner membrane vesicles wer...

The incorporation of labelled phosphate into the lipids of a brain dispersion.